Research reference · Suggested protocols

Suggested dosage protocols.

Educational reference for research-context dosing of every peptide in the Black Ice catalog. Doses shown below are aggregated from peer-reviewed literature, FDA drug labels (where applicable), and established research-supplier dosing pages. They are not treatment recommendations and are provided for laboratory and educational reference only.

Research use only · Not medical advice This page compiles publicly available research-context dosage protocols for educational and reference purposes. Products offered by Black Ice Peptides are sold strictly for in-vitro laboratory research and development. Nothing here should be interpreted as a prescription, treatment recommendation, or medical guidance. Any clinical or therapeutic use must be discussed with a licensed physician or qualified healthcare professional.
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TirzepatideRetatrutideBPC-157GHK-CuGHK-Cu SerumTB-500CJC-1295 (No DAC) + IpamorelinBPC + TB BlendPT-141GLOW Blend (BPC + TB + GHK-Cu)KLOW BlendIpamorelinCJC-1295 (No DAC)IGF-1 LR3TesamorelinKPVSemaxSemax Nasal SprayMOTS-cEpitalonNAD+SelankSelank Nasal SpraySemax + Selank Nasal SpraySemax + Selank BlendOxytocin Nasal SprayDSIPDSIP Nasal SprayAOD9604
BPC + TB Blend research vial
Blend · Repair

BPC + TB Blend

Typical dose250–500 mcg BPC-157 + 250 mcg–2.5 mg TB-500 (pre-blend: ~0.5–1 mg total daily)
FrequencyDaily (BPC-157); 2x/week (TB-500 loading), then 1–2x/week maintenance
RouteSubcutaneous
Cycle6–12 weeks total (4wk loading + 4-8wk maintenance), 2–4 week off-cycle
NotesPre-blended 1:1 vials (e.g., 10mg+10mg) dosed daily; or run on separate schedules
Sources
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KLOW Blend research vial
Blend

KLOW Blend

Typical dose2–4 mg total blend per injection (from 80mg vial: 50mg GHK-Cu/10mg BPC-157/10mg TB-500/10mg KPV)
FrequencyDaily, 5x/week loading (wk1-4), 3x/week maintenance (wk5-12)
RouteSubcutaneous
Cycle8–12 weeks on, 4–8 weeks off
NotesPer 10-unit (4mg) dose: ~2.5mg GHK-Cu + 500mcg each BPC-157/TB-500/KPV
Sources
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IGF-1 LR3 research vial
GH / IGF-1 axis

IGF-1 LR3

Typical dose20–50 mcg per injection
Frequency1x/day, post-workout or morning
RouteSubcutaneous (bilateral, rotate sites)
Cycle4–6 weeks on, 4–6 weeks off; do not run beyond 6 weeks continuous
NotesLong-acting Arg³ + N-terminal-extended IGF-1 analog with a ~20–30 hour half-life. Reconstitute with acetic acid or BAC water; unlike native IGF-1, LR3 resists binding-protein sequestration.
Sources
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Semax research vial
Neuro / cognitive

Semax

Typical dose300–1200 mcg/day (300–600mcg intranasal start; 200–400mcg subQ start)
Frequency1–2x/day (AM and early afternoon, 8-12hrs apart)
RouteIntranasal (primary) or subcutaneous
Cycle7–14 day titration then continued use; typically 2–4 week courses
NotesACTH(4-10) analog developed in Russia; nootropic via BDNF/dopamine pathways
Sources
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NAD+ research vial
Cellular energy

NAD+

Typical dose25–100 mg (subQ research dosing); 250–500 mg (IV clinic infusion)
FrequencyDaily or every other day (subQ); 1-2x/week loading then monthly (IV)
RouteSubcutaneous or intravenous
CycleNo established cycle length; often continuous or 4–8 week blocks
NotesStart at low end (25mg subQ) due to injection-site pain; NAD+ precursor for cellular metabolism research
Sources
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DSIP research vial
Sleep · Delta wave

DSIP

Typical dose100–300 mcg per injection
FrequencyNightly, 30–60 min before bed
RouteSubcutaneous
Cycle4–8 weeks, then reassess
NotesDelta-Sleep-Inducing Peptide; historical human research used 25–300 mcg/kg IV, but modern PT-style subcutaneous protocols report 100–300 mcg total per dose. Start low, titrate up if needed.
Sources
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Semax + Selank Blend vial
Neuro + Anxiolytic · Blend

Semax + Selank Blend

Typical dose300–600 mcg of each (Semax + Selank) per administration
Frequency1–2× daily (AM ± early PM)
RouteSubcutaneous (small volume) or intranasal after reconstitution
Cycle14–30 days on, then 7–14 days off
NotesCombines the standard Semax (300–600 mcg) and Selank (250–600 mcg) research doses in one blend. Avoid late-day dosing — Semax’s dopaminergic effect can interfere with sleep in some individuals.
Sources
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GHK-Cu 200mg Rejuvenation Serum
Cosmetic · Topical serum

GHK-Cu 200mg Rejuvenation Serum

Typical dose2–3 drops per application (approx. 5–10 mg GHK-Cu at 7% concentration)
FrequencyOnce or twice daily, AM and/or PM
RouteTopical — apply to clean, dry face and neck
CycleContinuous; visible results reported at 4–8 weeks, compounding through 12+ weeks
Notes7% clinical-strength GHK-Cu with 2% hyaluronic acid and 1% panthenol in an injection-grade water base. Refrigerate; use within 90 days of opening. Do not combine at the same time-of-day as high-dose vitamin C (destabilizes copper peptides) — alternate AM/PM.
Sources
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Semax Nasal Spray bottle
Nasal spray · Neuro / cognitive

Semax Nasal Spray

Typical dose3–6 sprays total (~300–600 mcg), split between nostrils
Frequency1–2× daily (AM, avoid late-day)
RouteIntranasal — 1 spray per nostril, alternate sides
Cycle14–30 days on, then 7–14 days off
NotesFormulated at 1 mg/mL, ~100 mcg per spray. Blow nose lightly before dosing. Tilt head slightly forward, spray while inhaling gently — do not sniff hard. Store refrigerated.
Sources
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Selank Nasal Spray bottle
Nasal spray · Anxiolytic

Selank Nasal Spray

Typical dose3–6 sprays total (~300–600 mcg), split between nostrils
Frequency1–3× daily as needed for stress/anxiety
RouteIntranasal — 1 spray per nostril, alternate sides
Cycle14–30 days on, then 7–14 days off (or acute use)
NotesFormulated at 1 mg/mL, ~100 mcg per spray. Non-sedating anxiolytic — safe to combine with Semax spray, offset dosing by 10–15 min if using both. Store refrigerated.
Sources
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Semax + Selank Nasal Spray research bottle
Nasal spray blend · Neuro + Anxiolytic

Semax + Selank Nasal Spray

Typical dose2–4 sprays/day total (1–2 per nostril per session, alternate nostrils)
Frequency1–2x/day (AM + early afternoon; last dose before 3pm to avoid sleep interference)
RouteIntranasal only
Cycle2–4 week courses on / 1–2 week off; sustained daily use above 4 weeks not typically recommended
NotesStack Spray — 10 mg Semax + 10 mg Selank in 10 mL sterile 0.9% saline. Pairs Semax's BDNF/nootropic activity with Selank's anxiolytic tone; store refrigerated after opening.
Sources
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Oxytocin Nasal Spray bottle
Nasal spray · Social / bonding

Oxytocin Nasal Spray

Typical dose4–8 IU (2–4 sprays total — ~100 mcg / ≈50 IU per spray)
Frequency1–2× daily or 30–45 min before social contexts
RouteIntranasal — 1 spray per nostril, alternate sides
CycleSituational or 2–4 weeks on, then break
NotesHuman research studies commonly use 24 IU (~48 mcg) doses; 1 mg/mL formulation delivers roughly 50 IU equivalent per spray. Effects on trust/social bonding are most cited in the 15–60 min post-dose window. Store refrigerated.
Sources
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DSIP Nasal Spray bottle
Nasal spray · Deep sleep

DSIP Nasal Spray

Typical dose2–4 sprays total (~200–400 mcg), split between nostrils
FrequencyNightly, 20–45 min before bed
RouteIntranasal — 1 spray per nostril, alternate sides
Cycle4–8 weeks, then reassess
NotesFormulated at 1 mg/mL, ~100 mcg per spray. Skip if using a heavy sedative same night — DSIP is subtle and stacks unpredictably with strong hypnotics. Store refrigerated.
Sources
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Full disclaimer The dosage information on this page is compiled from peer-reviewed publications, FDA drug labels (where applicable), and established peptide-research suppliers for the purpose of educational and research reference. Only two peptides on this list — Tesamorelin and PT-141 / Bremelanotide — have FDA-approved human dosing; all other protocols are drawn from research-context literature or extrapolated from animal studies via allometric scaling. Black Ice Peptides does not sell any product for human or veterinary consumption or administration. All Black Ice products are strictly for in-vitro laboratory research and development. This page does not constitute medical advice, treatment recommendation, prescription, or clinical guidance in any form. Always consult a licensed physician or qualified healthcare professional before any decision related to your health.