How to reconstitute your peptides.
Reconstitution means mixing your lyophilized (dry) research peptide with sterile bacteriostatic water so you can measure repeatable volumes on a U-100 insulin syringe. Use the calculator below to pick a BAC-water volume that gives you clean math for the concentration and dose you want to work with.
Reconstitution calculator
Pick your vial strength and how much BAC water you plan to add. We'll show the resulting concentration and the syringe reading for any dose.
Common vial charts
Download PDF cheat sheet| Vial | BAC water | Concentration | 0.25 mg dose | 0.5 mg dose | 1 mg dose |
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Most common combos
The setups researchers pick most often. All syringe reads assume a U-100 insulin syringe (100 units = 1 mL).
Choose your BAC volume
Bigger volume = lower concentration = easier syringe math. For a 10 mg vial, most researchers use 2 mL (5 mg/mL) or 2.5 mL (4 mg/mL). For a 5 mg vial, 1 mL (5 mg/mL) or 2 mL (2.5 mg/mL) are common.
Prep both vials
Wipe the rubber stopper on the peptide vial and the BAC water vial with 70% isopropyl alcohol and let it dry. Work on a clean, flat surface with everything you need already open.
Draw the BAC water
Use a 1 mL or 3 mL syringe with a drawing needle (18G–21G) to pull the exact BAC-water volume from the calculator. Keep the needle below the liquid line so you don't pull air.
Inject slowly, down the wall
Insert the needle into the peptide vial at an angle and inject the BAC water slowly down the inside wall of the vial. Never squirt directly onto the powder — the force can damage the peptide.
Swirl, don't shake
Gently roll or swirl the vial between your palms until the powder fully dissolves. This can take 30–90 seconds. Do not shake vigorously. The solution should be clear.
Label and refrigerate
Write the reconstitution date and concentration on the vial. Store at 36–46°F. Typical shelf life once reconstituted is 2–4 weeks. See the Storage page for peptide-specific data.